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CGP EDU Academic Team
Published on: August 13, 2026
Significance of 'heat shock' method in bacterial transformation is to facilitate
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Verified by ExpertsThe correct answer is:
C
In chemical method, the cell is treated with specific concentration of a divalent cation such as calcium to increase pore size in cell wall. The cells are incubated with recombinant DNA on ice, followed by placing them briefly at 
and then putting it back on ice. This is called heat shock method. The bacteria now takes up the recombinant DNA.
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